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Identification and characterization of dominant helper T-cell epitopes in the nucleocapsid protein of severe acute respiratory syndrome coronavirus

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Identification and characterization of dominant helper T-cell epitopes in the nucleocapsid protein of severe acute respiratory syndrome coronavirus

Auteurs : JINCUN ZHAO [République populaire de Chine] ; QIANRONG HUANG [République populaire de Chine] ; WEI WANG [République populaire de Chine] ; YAN ZHANG [République populaire de Chine] ; PING LV [République populaire de Chine] ; Xiao-Ming Gao [République populaire de Chine]

Source :

Journal of virology [ 0022-538X ] ; 2007.

RBID : Pascal:07-0279100

Descripteurs français

Pascal (Inist)
- Coronavirus, Identification, Cellule helper, Lymphocyte T, Déterminant antigénique, Nucléocapside, Protéine, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Antigenic determinant, Coronavirus, Helper cell, Identification, Nucleocapsid, Protein, Severe acute respiratory syndrome, T-Lymphocyte, Virology.

Abstract

By using a series of overlapping synthetic peptides covering 98% of the amino acid sequence of the nucleocapsid protein (NP) of severe acute respiratory syndrome coronavirus (SARS-CoV), four helper T-cell (Th) epitopes (NP11, residues 11 to 25; NP51, residues 51 to 65; NP61, residues 61 to 75; and NP111, residues 111 to 125) in C57BL mice (H-2^b), four (NP21, residues 21 to 35; NP91, residues 91 to 105; NP331, residues 331 to 345; and NP351, residues 351 to 365) in C3H mice (H-2^k), and two (NP81, residues 81 to 95; and NP351, residues 351 to 365) in BALB/c mice (H-2^d) have been identified. All of these peptides were able to stimulate the proliferation of NP-specific T-cell lines or freshly isolated lymph node cells from mice immunized with recombinant NP. Immunization of mice with synthetic peptides containing appropriate Th epitopes elicited strong cellular immunity in vivo, as evidenced by delayed-type hypersensitivity. Priming with the helper peptides (e.g., NP111 and NP351) significantly accelerated the immune response induced by recombinant NP, as determined by the production of NP-specific antibodies. When fused with a conserved neutralizing epitope (SP1143-1157) from the spike protein of SARS-CoV, NP111 and NP351 assisted in the production of high-titer neutralizing antibodies in vivo. These data provide useful insights regarding immunity against SARS-CoV and have the potential to help guide the design of peptide-based vaccines.

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">By using a series of overlapping synthetic peptides covering 98% of the amino acid sequence of the nucleocapsid protein (NP) of severe acute respiratory syndrome coronavirus (SARS-CoV), four helper T-cell (Th) epitopes (NP11, residues 11 to 25; NP51, residues 51 to 65; NP61, residues 61 to 75; and NP111, residues 111 to 125) in C57BL mice (H-2<sup>b</sup>
), four (NP21, residues 21 to 35; NP91, residues 91 to 105; NP331, residues 331 to 345; and NP351, residues 351 to 365) in C3H mice (H-2<sup>k</sup>
), and two (NP81, residues 81 to 95; and NP351, residues 351 to 365) in BALB/c mice (H-2<sup>d</sup>
) have been identified. All of these peptides were able to stimulate the proliferation of NP-specific T-cell lines or freshly isolated lymph node cells from mice immunized with recombinant NP. Immunization of mice with synthetic peptides containing appropriate Th epitopes elicited strong cellular immunity in vivo, as evidenced by delayed-type hypersensitivity. Priming with the helper peptides (e.g., NP111 and NP351) significantly accelerated the immune response induced by recombinant NP, as determined by the production of NP-specific antibodies. When fused with a conserved neutralizing epitope (SP1143-1157) from the spike protein of SARS-CoV, NP111 and NP351 assisted in the production of high-titer neutralizing antibodies in vivo. These data provide useful insights regarding immunity against SARS-CoV and have the potential to help guide the design of peptide-based vaccines.</div>
</front>
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<name sortKey="Qianrong Huang" sort="Qianrong Huang" uniqKey="Qianrong Huang" last="Qianrong Huang">QIANRONG HUANG</name>
<name sortKey="Wei Wang" sort="Wei Wang" uniqKey="Wei Wang" last="Wei Wang">WEI WANG</name>
<name sortKey="Yan Zhang" sort="Yan Zhang" uniqKey="Yan Zhang" last="Yan Zhang">YAN ZHANG</name>
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Identification and characterization of dominant helper T-cell epitopes in the nucleocapsid protein of severe acute respiratory syndrome coronavirus

Identification and characterization of dominant helper T-cell epitopes in the nucleocapsid protein of severe acute respiratory syndrome coronavirus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri